TP-024

Protein target name:

GPR52

Mechanism of action:

Agonist

Recommended Concentration for use in cells:

1 uM

Probe Information

Target and Probe Information

Target Details

Target class:

GPCR

Subclass:

A orphan G protein-coupled receptor

HUGO ID:

HGNC:4508

Entrez gene ID:

9293

UNIPROT ID:

Q9Y2T5

Probe Details

Chemical Probes Portal ID:

CPP1834

Probe alias:

FTBMT

PubChem CID:

56649300

ChEMBL ID:

CHEMBL4082756

SMILES string:

Cc1cc(ccc1C(=O)N)n2nc(Cc3cc(F)cc(c3)C(F)(F)F)nc2C

InChi Key:

TYXSIXOYTBHZFA-UHFFFAOYSA-N

Control compound:

TP-024n

Physico-chemical properties

Solubility - preferred solvent:

DMSO

Solubility - concentration:

10 mM

NMR:

1H NMR (500 MHz, DMSO-d6) - δ 7.83 (s, 1H), 7.61 (s, 1H), 7.52 (m, 3H), 7.48 (s, 1H), 7.47 – 7.41 (m, 2H), 4.16 (s, 2H), 2.45 (sd, J = 1.0 Hz, 3H), 2.43 (s, J = 1.3 Hz, 3H)

13C NMR (126 MHz, DMSO-d6) - δ 170.58, 163.35, 161.39, 161.14, 153.46, 143.27, 143.21, 137.90, 137.50, 137.45, |2J (C, F)|= 131.73-,130.88, 128.60, |1J (C, F)|= 127.11-120.63, 126.47, 122.35, 122.32, 122.29, 121.62, 120.64, 120.48, |1J (C, F)|= 111.44-111.18, 33.57, 19.93, 13.39

Storage Recommendations

Storage (solution):

-20

Validation in Cells and Model Organisms

In cell validation

On target activity in cells

Potency in cells:

IC50

93 nM in human141 nM in Mouse134 nM in Rat

Potency assay (cells):

CHO-Bases cAMP assay

Off target activity in cells

Probe Selectivity in Cell:

No significant activity at 10 μM.

In vivo validation

Organism:

Rat, Mouse, Human

Dose:

10 to 30 mg/Kg in Rat

Route of delivery:

Oral

Cmax:

0.688 ug/ml

Tmax:

0.5 h 1 h

Primary Reference

Reference (doi):

10.1016/j.bmc.2017.03.064 10.1124/jpet.117.242925

Reference (PMID):

28433511 28851764

External Resources

Available data:

SGC additional analysis

TP-024

Probe Information

Target Details

Probe Details

Physico-chemical properties

In cell validation

In vivo validation

Primary Reference

External Resources

Supporting Organizations

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TP-024

Probe Information

Target Details

Probe Details

Physico-chemical properties

In cell validation

In vivo validation

Primary Reference

External Resources

Supporting Organizations

Interested in supporting the portal?