PF-4181366

Protein target name:

PDE9A

Mechanism of action:

Inhibitor

Probe Information

Target and Probe Information

Target Details

Target class:

Nucleic acid metabolism

Subclass:

Phosphodiesterase

HUGO ID:

HGNC:8795

Entrez gene ID:

5152

UNIPROT ID:

O76083

Target alias:

PDE9, HSPDE9A2

Probe Details

Chemical Probes Portal ID:

CPP1680

Probe alias:

cpd 19

PubChem CID:

25116766

ChEMBL ID:

CHEMBL583914

SMILES string:

O=C1C2=C(N(C3CCCC3)N=C2)N=C([C@@H]4CN(CC5=CC=C6N=CC=NC6=C5)C[C@H]4C)N1

InChi Key:

SNCRCEGCQVMUBS-DNVCBOLYSA-N

Physico-chemical properties

NMR:

1H NMR 400 MHz(CDCl3) δ 8.82 (s, 2H), 8.14 (d, J = 8.3 Hz, 1H), 8.07-7.93 (m, 3H), 5.15-5.08 (m, 1H), 4.00-3.87 (m, 2H), 3.37 (t, J = 8.7 Hz, 1H), 3.04 (d, J = 9.5 Hz, 1H), 2.85-2.84 (m, 1H), 2.66-2.62 (m, 5 1H), 2.46-2.42 (m, 1H), 2.11-1.92 (m, 7H), 1.71-1.63 (m, 2H), 1.20 (d, J= 7.05 Hz, 3H). MS: (M+H m/z = 430.1).

Validation in Cells and Model Organisms

In vivo validation

Ratio of probe in organ to plasma (O:P):

1:4

Organ of Interest

Organ (O):

Brain

Target engagement assay:

PF-4181366 elevated cGMP in the striatum of mice in a dose responsive fashion. A 145% elevation occurred at 3.2 mg/kg, and at 10 mg/kg a 215% elevation was observed.

Primary Reference

Reference (doi):

10.1021/jm9015334

Reference (PMID):

19919087

PF-4181366

Probe Information

Target Details

PDE9, HSPDE9A2

Probe Details

Physico-chemical properties

In vivo validation

Primary Reference

Supporting Organizations

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PF-4181366

Probe Information

Target Details

PDE9, HSPDE9A2

Probe Details

Physico-chemical properties

In vivo validation

Primary Reference

Supporting Organizations

Interested in supporting the portal?